Skip to main content
. Author manuscript; available in PMC: 2023 Sep 7.
Published in final edited form as: Nature. 2021 Jun 2;594(7863):430–435. doi: 10.1038/s41586-021-03525-z

Figure 2. Notum is required for Apc-mutant fixation in vivo.

Figure 2

a, Representative β-catenin IHC reveals fully and partially Apc-mutant fixed crypts in Lgr5CreER;Apcfl/fl (Notum+/+) and Lgr5CreER;Apcfl/fl;Notumfl/fl (Notumfl/fl) mice. n=4 mice per genotype were stained. Scale bar, 200 μm. b, Ratio of fully-to-partially fixed crypts in Notum+/+ and Notumfl/fl mice induced with 0.15 mg tamoxifen and sampled at 10, 14, and 21 days post induction (d.p.i). n=4 mice/genotype per timepoint. c, Total number of nuclear β-catenin+ lesions in Notum+/+ and Notumfl/fl mice over 10, 14, and 21 days post induction. n=4 mice/genotype per timepoint. d, Immunofluorescence staining for β-catenin (red) and lysozyme (green) of representative whole-mount Apc-mutant clones (red) from Notum+/+ and Notumfl/fl mice 21 days post induction. Nuclei were labelled with DAPI (blue). n=4 mice per genotype were stained. Scale bar, 200 μm. e, Left, heat maps depict the relative frequency of mutant clones of the indicated size (columns) at various timepoints (rows) for both Notum+/+ and Notumfl/fl mice. Right, graph displays the average clone size of Notum+/+ and Notumfl/fl mice over time post tamoxifen-induction. n=4 mice/genotype per timepoint. Data are mean ± s.e.m. In b, c, e, Mann–Whitney two-tailed U-test; P values are shown in the corresponding panels.