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. Author manuscript; available in PMC: 2023 Sep 8.
Published in final edited form as: Nat Cardiovasc Res. 2022 Sep 9;1:806–816. doi: 10.1038/s44161-022-00125-6

Figure 1. Cre-LoxP mediated recombination of target genes.

Figure 1

Following translocation to the nucleus, Cre recombines loxP sites that have been engineered into the mouse genome, thereby excising the intervening sequences. a, The Cre–LoxP system can be used to delete a critical exon in a floxed gene. b, The Cre–LoxP system can delete a floxed stop codon to activate the expression of a reporter gene, which allows monitoring of Cre activity and genetic lineage tracing. c, The CreER fusion protein is retained in the cytoplasm until 4- hydroxytamoxifen (OHT) binding induces nuclear translocation, termed CreER activation, for example to remove a stop codon in front of a reporter.