(A) NMuMG cells were transfected with non-targeting (NT) control siRNAs or siRNAs targeting Vps28 and treated or not with 0.5 ng/mL TGF-β for the times indicated. Cells were fixed and stained for TJP1, CDH1, and DAPI to mark nuclei and imaged by confocal microscopy. Scale bar, 50 μm.
(B) As in (A) except that cells were stained for actin using phalloidin. Scale bar, 30 μm.