Figure 5. Pathogenicity-induced SSPs (PiSSPs) of A. luteobubalina induce cell death in host plants.
a) Heatmap shows log2 fold changes for SSPs significantly upregulated in at least one time point. Red shows higher and blue depicts lower logFC, followed by presence/absence matrix of homologs of unannotated SSPs in 131 species (Dataset 2). The two experimentally validated PiSSPs are shown by red arrows. b) Transient transformation of the non-host N. benthamiana and the host E. grandis with negative controls (empty vector; Pisolithus microsporus proteins Pismi_683611 and Pismi_689601), with a positive control (BAX) or with an in planta expression vector encoding Armlut_1165297 or Armlut_1348401. As further controls, frameshift versions of the A. luteobubalina sequences were included (Armlut_1165297_fs or Armlut_1348401_fs).