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. Author manuscript; available in PMC: 2023 Nov 10.
Published in final edited form as: Nat Chem. 2023 Jun 15;15(7):948–959. doi: 10.1038/s41557-023-01232-y

Fig. 1. Relationship between sequence identity and cross-reactivity in previously characterized ΔN PylRSs and pyl tRNAs.

Fig. 1

a. Activity of each combination of ΔN PylRSi and ΔN tRNAPylj, measured by production of GFP(150AllocK)His6 from cells bearing a GFP(150TAG)His6 gene in the presence of 4 mM AllocK 1, plotted against the sequence identity between ΔN PylRSi and ΔN PylRSj, where ΔN PylRSj is the synthetase from the same organism as ΔN tRNAPylj. ΔN PylRS proteins with greater than 55% sequence identity (dashed grey line) are predominantly active with each other’s pyl tRNAs (88% of cases). ΔN PylRS proteins with less than 55% sequence identity may or may not be active with each other’s pyl tRNAs. b. Activity of each combination ΔN PylRS with greater thani and ΔN tRNAPylj, plotted against the sequence identity between ΔN tRNAPyli and ΔN tRNAPylj, where ΔN tRNAPyli is the tRNAPyl from the same organism as ΔN PylRSi. ΔN pyl tRNAs with greater than 75% sequence identity (dashed grey line) are predominantly active with each other’s synthetases (93% of cases). ΔN pyl tRNAs with less than 75% sequence identity may or may not be active with each other’s synthetases. Dots represent the mean of three biological replicates, error bars are shown in Supplementary Figure 1. All numerical values are provided in Supplementary Table 2.