Table 3.

Nucleotide sequence of the primers used for amplification of HEV G3 and norovirus GII by semi-nested PCR

Assay	Primer name and nucleotide sequences	Amplicon size (bp)	Target fragment size (bp)b	Reference
Norovirus GII	First round QNIF2D: 5′-ATGTTCAGRTGGATGAGRTTCTCWGA-3′ GIISKR: 5′-CCRCCNGCATRHCCRTTRTACAT-3′	378		Loisy et al., (2005); Kojima et al., (2002)
	Second round GIISKF_T: 5′-TTTCTGTTGGTGCTGATATTGCCNTGGGAGGGCGATCGCAA-3′ GIISKR_T: 5′-ACTTGCCTGTCGCTCTATCTTCCCRCCNGCATRHCCRTTRTACAT-3′	344a	302	Kojima et al., (2002)
HEV G3	First round G3STF1: 5′-TGTTGCGCAGGTYTGTGT-3′ G3STR1: 5′-GCARCATAGGCARAARCACGA-3′	258		This study
	Second round G3STF2: 5′-TTTCTGTTGGTGCTGATATTGCTGTTGCGCAGGTYTGTGT-3′ G3STR2: 5′-ACTTGCCTGTCGCTCTATCTTCCATAGGCARAARCACGARGAA-3′	254a	215	This study

^aThe amplicon size given here does not include the primer adapters, which added 44 more base pairs. Sequences in bold are the primer adapters

^bThe amplicons are trimmed to remove the primer sequences to give the trimmed target fragment