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. Author manuscript; available in PMC: 2023 Dec 4.
Published in final edited form as: Dev Cell. 2023 Sep 9:S1534-5807(23)00436-7. doi: 10.1016/j.devcel.2023.08.021
Reagent or Resource Source Identifier
Antibodies
Anti-phospho-Smad2 (Zebrafish IF, Dilution: 1:500) Cell Signaling Technology Cat # 8828; RRID: AB_2631089
Anti-diphospho-ERK (Zebrafish IF, Dilution: 1:500) Sigma Cat # M8159; RRID: AB_477245
Anti-diphospho-ERK (Drosophila IF, 1:200) Cell Signaling Technology Cat #9101; RRID: AB_331646
Anti-phospho-histone H3 (Zebrafish IF, Dilution: 1:1000) Abcam Cat#ab183626
Anti-phospho-histone H3 (Zebrafish IF, Dilution: 1:500) Cell Signaling Technology Cat # 9706, RRID: AB_331748
HRP-conjugated anti-mouse secondary antibodies (IF, Dilution: 1:500) Dako Cat # P0447 RRID: AB_2617137
HRP-conjugated anti-rabbit secondary antibodies (IF, Dilution: 1:500) Dako Cat # P0448 RRID: AB_2617138
Chemicals, peptides, and recombinant proteins
Tyramide hydrochloride Sigma Cat # T2879
NHS-Fluorescein ester ThermoFisher Scientific Cat #46410
Cy3 mono NHS ester Sigma Cat# PA13101
Cy5 mono NHS ester Sigma Cat# PA15101
PD-0325901 Merck Cat # 444968
RO-3306 Sigma Cat #217721
Cyclosporin A Selleck Cat # S2286
DAPI Sigma Cat# 10236276001
FuGene Promega Cat # E269A
Fetal Bovine Serum Thermofisher Scientific Cat# 10270-106
DMEM/F-12 ThermoFisher Scientific Cat# 10565018
Halocarbon oil 27 Sigma Cat # H8773
Halocarbon oil 700 Sigma Cat # H8898
Critical Commercial Assays
Multiplex Fluorescent Assay v2 ACDBio acdbio.com
Experimental models: Cell Lines
NIH-3T3 cells, mouse Francis Crick Institute Cell Services N/A
Experimental models: Organisms/Strains
Zebrafish Danio rerio : WT Francis Crick Aquatics N/A
Zebrafish Danio rerio : tg(ubiPErk-KTR-Clover) Mayr et al.9 N/A
Zebrafish Danio rerio : tg(ubiP:modErk-KTR-Clover) This paper N/A
Drosophila melanogaster: P{y[+t7.7] w[+mC]=UAS-ERK-KTR-T2A-H2Av-mCh} attP64/TM3, Sb[1] Yuen et al.34 BDSC:93895
Drosophila melanogaster: y[1] w[*];P{y[+t7.7] w[+mC]=UAS-modERK-KTR-T2A-H2Av-mCh}attP40/CyO This paper BDSC:95286
Drosophila melanogaster: y[1] w[*]; P{y[+t7.7] w[+m*]=nanosP-ERK-KTR-T2A-H2Av-mCh}attP2/TM3, Sb[1] This paper N/A
Drosophila melanogaster: y[1] w[*]; P{y[+t7.7] w[+m*]=nanosP-modERK-KTR-T2A-H2Av-mCh}attP2/TM3, Sb[1] This paper BDSC:95288
Recombinant DNA
pCS2-Tol2 recombinase Kawakami et al.63 N/A
pCS2-mScarletI-H2B This paper N/A
pCS2-fgf8a van Boxtel et al.16 N/A
pCS2- XdnFGFR Amaya et al.64 N/A
pDEST- ubiP : ERK-KTR-Clover-pA-Tol2 Mayr et al.9 N/A
pDEST- ubiP : modErk-KTR-Clover-pA-Tol2 This paper N/A
pUASt - modERK-KTR-T2A-H2Av- mCherry-attB This paper N/A
pNosP - ERK-KTR-T2A-H2Av-mCherry-attB This paper N/A
pNosP - modERK-KTR-T2A-H2Av- mCherry-attB This paper N/A
pCS2-modErk-KTR-Clover This paper N/A
pCS2-modErk-KTRAAA-Clover This paper N/A
pCS2-modErk-KTREEE-Clover This paper N/A
Software and algorithms
FIJI (ImageJ) Schneider et al.65 https://imagej.net/Fiji/Downloads
Prism GraphPad https://www.graphpad.com/scientific-software/prism/
Other
Drgsc-C3 (RNAscope) ACDbio Cat # 427301-C3
Drfgf3-C4 (RNAscope) ACDbio Cat # 850161-C4
Drfgf8a-C2 (RNAscope) ACDbio Cat # 559351-C2
35 mm Petri dish, 14 mm microwell No. 1.5 coverglass MatTek Life Sciences Cat# P35G-1.5-14-C
lumox® dish with foil base, 0: 50 mm Sarstedt AG & Co Cat # 94.6077.305
Coverslip No. 1 18x18mm Scientific Laboratory Supplies Cat# MIC3110
Coverslip No. 0 18x18mm Scientific Laboratory Supplies Cat# MIC3100
Coverslip No. 1.5 24x40mm Scientific Laboratory Supplies Cat # MIC3252