Skip to main content
. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: Cell Host Microbe. 2023 Mar 29;31(4):604–615.e4. doi: 10.1016/j.chom.2023.03.004

Figure 4. Characterization of VP4 in the eDLP assembly intermediate.

Figure 4

(A) Overview of the eDLP VP4 density. The VP6 layer is colored in orange with two of the subunits hidden to expose the foot domains of VP4. The membrane layer is represented as a cartoon. Insets show density in the lobes, struts, and foot regions.

(B) Flexibility analysis of the pre-mature spike is depicted as central sections through each of the densities. The number of particles in each class is highlighted in the histogram.

(C) Map of VP4 after focused classification following multibody analysis. Fit of one of the chains of the pre-mature VP4, colored as in Figure 3A. The membrane and two VP6 subunits have been masked out to aid visualization. The approximate location of the membrane is depicted as a cartoon (gray).

(D) Liquorice diagram model of the pre-mature VP4 trimer.

(E) Comparison of the pre-mature (left) and extended (right) VP4 molecules. VP4-A and B chains are rendered as surfaces, with VP4-C represented as a liquorice cartoon.

(F) Comparison of VP4 from the entry intermediate form of the TLP (PDB: 6wxf, right) with the pre-mature VP4 seen on the eDLP (left). The different protein layers are represented as liquorice cartoons. Red triangle indicates the 3-fold axis.