Figure 2.
Assessment of gene expression thresholds using single-copy knockin reporters
GFP-positive neurons were identified using the NeuroPAL multicolor transgene.69 Segments showing neuronal expression are pictured; individual neurons are labeled. Scale bars represent 10 μm.
(A) Strategy for neuron identification. Reporter expression is overlaid with the multicolor NeuroPAL expression pattern, allowing neuron identification.
(B) Reporters for representative NPP genes nlp-45 (left) and flp-20 (right).
(C) Reporters for representative GPCR genes tkr-1 (above) and dmsr-6 (below). Images for the complete set of 17 NPP and nine GPCR reporters are in Figure S1.