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. Author manuscript; available in PMC: 2024 Jan 11.
Published in final edited form as: Small. 2022 Jun 30;18(36):e2202303. doi: 10.1002/smll.202202303

Figure 3.

Figure 3

a) Cumulative release of pCAG-GFP plasmid DNA (nanograms) versus time (hours) and b) total amount of pCAG-GFP released at 24 h from 8 × 8 mm silicon substrates coated with hyaluronate-chitosan nanofilms containing 1 to 5 layers of polyplexes (1P to 5P) in PBS buffer pH 7.4 containing 10 mM of reduced glutathione at 37 °C. Points and bars represent the mean of three replicates (N = 3) ± SEM. Statistical significance as (*) p < 0.05 and (**) p < 0.01, using one-way ANOVA with Sidak’s test to compare 1P to 4P. (ns) non-significant difference. 5P was not considered in the analysis. Linear trend post-test with p < 0.0001. c) Nanofilm degradation over time (hours) in function of surface topography via AFM assessments. Scale bars represent 2 μm.