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. 2023 Aug 2;11(3):101065. doi: 10.1016/j.gendis.2023.06.034

Figure 3.

Figure 3

LKB1 depletion leads to autophagy inhibition-mediated EMT via the p62-NFκB-Snail2 pathway in ATII cells. (A) Protein expression of LC3-I, LC3-II, p62, and LKB1 in ATII cells transfected with the indicated siRNA. β-actin was used as a loading control. (B) Immunofluorescence staining of p62 (red) in ATII cells transfected with the indicated siRNA. DAPI (blue) was used to stain nuclei. Scale bar, 40 μm. (C) NF-κB reporter assays in ATII cells with the indicated treatment. Values represent the relative fold of Renilla luciferase, normalized against control (1.0). The data were expressed as mean ± standard deviation. n = 3 samples in each group; ∗∗P < 0.01. (D) Protein expression of Snail2, p65, and LKB1 in ATII cells with the indicated treatment. β-Tubulin was used as a loading control. (E) Protein expression of Snail2, p62, and LKB1 in ATII cells with the indicated treatment. β-actin was used as a loading control.