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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Neuron. 2022 Sep 28;110(23):3936–3951.e10. doi: 10.1016/j.neuron.2022.09.002

Figure 1. Human developing brain organotypic slices are vulnerable to ZIKV whereas GBM slices are refractory.

Figure 1

(A) Primary HDB and GBM samples were processed for slice culture, then infected with ZIKV PE243.

(B) RNAscope smFISH of representative ZIKV-infected HDB and GBM slice cultures with ZIKV 1x10^7 PFU, 72 hours post infection (h.p.i.), using SOX2 (red) and ZIKV (green) probes. White arrow denote ZIKV infection.

(C) smFISH of ZIKV-infected HDB and GBM slice cultures with ZIKV 1x10^7 PFU, 7 days post infection (d.p.i.), using ZIKV probe (green).

(D) RT-qPCR of ZIKV in HDB and GBM slice cultures, infected with ZIKV 1x10^7 PFU, 7 d.p.i., (Median and interquartile range indicated. **** Mann Whitney U t-test p <0.0001). Data points represent replicate slices from HDB n=2 specimens, both with hb and fb regions, and GBM n=3 patients.