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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Neuron. 2022 Sep 28;110(23):3936–3951.e10. doi: 10.1016/j.neuron.2022.09.002

Figure 6. Glioma myeloid cells drive Zika resistance through cytokine secretion.

Figure 6

(A) Conditioned media harvested from GBM11b+ fractions (11b+CM), from GBM11b-fractions (11b-CM) and from GBM lines (LineCM), profiled using Meso Scale Discovery U-Plex and V-Plex (MSD) cytokine panels, and applied to GBM lines for conditioned media functional assays and expression profiling.

(B) ZIKV RT-qPCR 48 hours post infection (MOI = 1) of GBM lines GBM E22 and GBM E34 and HDB FB1 cells cultured in base media, LineCM, 11b-CM or 11b+CM. (2-way ANOVA cell line conribtion p < 0.0001 and conditioned media contribution p < 0.0001; Welch’s t-test Base media vs 11b+CM *** p<0.001, ** p<0.01)

(C) Venn diagram summarises pattern of gene upregulation in GBM E22, GBM E34, and primary HDB cells (specimen BRC 2251) post-treatment with 11b+CM versus control LineCM.

(D) MSD assay of cytokine content in 11b-CM and 11b+CM at 48 h in culture (n=4 paired GBM sample fractions from 4 patient tumours).

(E) MSD assay of Pi:c-LineCM and Pi:c-11b+CM derived from the HDB and GBM fractions indicated, following stimulation with poly(I:C) viral dsRNA mimetic at 10 μg/ml.