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. Author manuscript; available in PMC: 2024 Feb 12.
Published in final edited form as: Sci Immunol. 2023 Sep 29;8(87):eadd1599. doi: 10.1126/sciimmunol.add1599

Figure 8. IL-17A+Roryt+ γδ T cells in blood are a potential non-invasive biomarker for human NASH.

Figure 8

(a) WT mice were fed with a NCD or SSD diet. At indicated time points, blood leukocytes were restimulated in vitro with PMA/Ionomycin and IL-17A production by γδ T cells was measured by flow cytometry (n=5). (b-c) human patients with MAFLD were analyzed for liver stiffness (LSM) by ultrasound measurement. Next, their peripheral blood mononuclear cells were restimulated in vitro with PMA/Ionomycin and γδ T cells were analyzed by flow cytometry. (b) correlation between liver stiffness measurement (LSM; an indicator of fibrosis) and IL-17A production by γδ T cells (n=18). (c) correlation between LSM and RORγt expression by γδ T cells (n=18). Shown are means +/- s.e.m. Statistical significance was determined by unpaired t-test (a), or by linear regression (b,c). Statistical significance was defined as *p<0.05; **p<0.01; ***p<0.001.