| Armenian hamster anti-mouse monoclonal antibody TCR gamma/delta (GL3) |
eBioscience |
17-5711-82 |
| Armenian hamster anti-mouse monoclonal antiobody TCR beta (H57-597) |
eBioscience |
12-5961-82 |
| Rat anti-mouse CD4 (RM4-5) |
eBioscience |
63-0042-82 |
| Armenian hamster anti-mouse CD3e monoclonal antibody (Clone 145-2C11) |
eBioscience |
12-0031-82 |
| Armenian hamster anti-mouse CD69 monoclonal antibody (Clone H1.2F3) |
eBioscience |
11-0691-81 |
| Armenian hamster anti-mouse gdTCR monoclonal antibody (Clone GL-3) |
eBioscience |
17-5711-82 |
| Fixable Viability Dye eFluor™ 506 |
eBioscience |
65-0865-14 |
| Rat anti-mouse CD8a monoclonal antibody (53-6.7) |
eBioscience |
48-0081-82 |
| Rat anti-mouse CD19 monoclonal antibody (1D3) |
eBioscience |
78-0193-82 |
| Rat anti-mouse IL-17A monoclonal antibody (eBio17B7) |
eBioscience |
25-7177-82 |
| Mouse anti-mouse CD45.2 monoclonal antibody (Clone 104) |
eBioscience |
47-0454-82 |
| Mouse anti-mouse IFNγ monoclonal antibody (Clone AN-18) |
eBioscience |
14-7313-81 |
| Rat anti-mouse IFNg monoclonal antibody (XMG1.2) |
eBioscience |
12-7311-82 |
| Rat anti-mouse TNF monoclonal antibody (MP6-XT22) |
eBioscience |
53-7321-82 |
| Rat anti-mouse CD49b monoclonal antibody (DX5) |
eBioscience |
13-5971-82 |
| Armenian hamster anti-mouse CD27 monoclonal antibody (LG.7F9) |
eBioscience |
12-0271-82 |
| Rat anti-mouse CD11b monoclonal antibody (M1/70) |
eBioscience |
25-0112-82 |
| Mouse anti-mouse NK1.1 monoclonal antibody (PK136) |
eBioscience |
47-5941-82 |
| Rat anti-mouse CD335 (Nkp46) monoclonal antibody (29A1.4) |
eBioscience |
11-3351-82 |
| Rat anti-mouse CD68 monoclonal antibody (FA-11) |
eBioscience |
25-0681-82 |
| Rat anti-mouse CD68 monoclonal antibody (FA-11) |
eBioscience |
25-0681-82 |
| Armenian hamster anti-mouse CD11c monoclonal antibody (N418) |
eBioscience |
63-0114-82 |
| Ly-6G |
eBioscience |
48-9668-82 |
| Rat anti-mouse Siglec F monoclonal antibody (1RNM44N) |
eBioscience |
46-1702-82 |
| Rat anti-mouse Ly6G monoclonal antibody (1A8-Ly6g) |
eBioscience |
48-9668-82 |
| Rat anti-mouse Ly6C monoclonal antibody (HK1.6) |
eBioscience |
12-5932-82 |
| Rat anti-mouse MHC II monoclonal antibody (M5/114.15.2) |
eBioscience |
11-5321-85 |
| Rat anti-mouse F4/80 (BM8) |
eBioscience |
17-4801-82 |
| CD314 (NKG2D) (CX5) |
eBioscience |
25-5882-82 |
| Rat anti-mouse/human/rhesus monkey ROR gamma (t) monoclonal antibody (AFKJS-9) |
eBioscience |
61-6988-82
|
| Mouse anti mouse/human/rhesus monkey T-bet monoclonal antibody (4B10) |
eBioscience |
12-5825-82 |
| Rat anti-mouse/human Eomes monoclonal antibody (Dan11mag) |
eBioscience |
53-4875-82 |
| Armenian hamster anti-mouse TCRVγ1 monoclonal antibody (2.11) |
Biolegend |
141105 |
| Armenian hamster anti-mouse TCRVγ2 (UC3-10A6) |
Biolegend |
137703 |
| Sirian hamster anti-mouse TCRVγ3 (536) |
Biolegend |
137503 |
| Mouse anti-mouse TCRVγ4 monoclonal antibody (UC3-10A6) |
Biolegend |
160602 |
| Rat anti-mouse CD122 monoclonal antibody (TM-b1) |
eBioscience |
11-1222-82 |
| Rat anti-mouse CD44 monoclonal antibody (IM7) |
eBioscience |
47-0441-82 |
| Mouse anti- mouse/human/mammal/rat CD25 monoclonal antibody (CD25-3G10) |
eBioscience |
MA1-35144 |
| Armenian hamster anti-mouse CD69 (H1.2F3) |
eBioscience |
25-0691-82 |
| Rat atni-mouse CD127 monoclonal antibody (A7R34) |
eBioscience |
12-1271-82 |
| Rat anti-mouse CD16/32 monoclonal antibody (93) |
eBioscience |
16-0161-82 |
| Mouse anti-mouse CD45.2 monoclonal antibody (104) |
eBioscience |
56-0454-82 |
| Rat anti-mouse IgM (II/41) |
eBioscience |
12-5790-82 |
| Rat anti-dog/monkey/human/mouse Ki-67 monoclonal antibody (SolA15) |
eBioscience |
11-5698-82 |
| Rat anti-mouse CD206 monoclonal antibody (MR5D3) |
BD Biosciences |
MA5-16870 |
| Mouse anti-human gdTCR monoclonal antibody (B1.1) |
eBioscience |
TCR2720 |
| Mouse anti-human CD161 (HP-3G10) |
eBioscience |
12-1619-42 |
| Rat anti-mouse ROR gamma t monoclonal antibody (B2D) |
eBioscience |
12-6981-82 |
| Mouse anti-human NKG2D monoclonal antibody (1D11) |
eBioscience |
46-5878-42 |
| Mouse anti-human CD45 monoclonal antibody (HI30) |
eBioscience |
MHCD4531 |
| Mouse anti-human IL-17A monoclonal antibody (eBio64DEC17) |
eBioscience |
17-7179-42 |
| Mouse anti-human CD8 monoclonal antibody (OKT8) |
eBioscience |
47-0086-42 |
| Mouse anti-human CD4 monoclonal antibody (OKT4) |
eBioscience |
63-0048-42
|
| Mouse anti-human CD3 monoclonal antibody (OKT3) |
eBioscience |
67-0037-42 |
| Vα7.2 (REA179) |
Miltenyi |
130-100-188 |
| Rat anti-mouse IFNγ (biotinylated)monoclonal antibody (Clone R4-6A2) |
eBioscience |
13-7312-81 |
| Rat anti-mouse IFNβ monoclonal antibody (Clone 7F-D3) |
abcam |
ab24324 |
| Rat anti-mouse TNFα monoclonal antibody (Clone MP6-XT22) |
eBioscience |
11-7321-82 |
| Rat anti-mouse CD11b monoclonal antibody (M1/70) |
BD |
612800 |
| Rat anti-mouse CD11b monoclonal antibody (M1/70) |
Biolegend |
101239 |
| Armenian hamster anti-mouse CD11cmonoclonal antibody (N418) |
eBioscience |
35-0114-82 |
| Rat anti-mouse CD127 monoclonal antibody (A7R34) |
Biolegend |
135027 |
| Rat anti-mouse CD16/32 monoclonal antibody (93) |
Biolegend |
101301 |
| Rat anti-mouse CD172a monoclonal antibody (P84) |
BD |
740282 |
| Rat anti-mouse CD19 monoclonal antibody (1D3) |
BD |
612971 |
| Rat anti-mouse CD206 monoclonal antibody (C068C2) |
Biolegend |
141734 |
| Armenian hamster anti-mouse CD27monoclonal antibody (LG.3A10) |
Biolegend |
124216 |
| Rat anti-mouse CD3 monoclonal antibody (17A2) |
Biolegend |
100220 |
| Rat anti-mouse CD4 monoclonal antibody (GK1.5) |
Biolegend |
100473 |
| Rat anti-mouse CD44 monoclonal antibody (IM7) |
BD |
612799 |
| Rat anti-mouse CD45 monoclonal antibody (30-F11) |
Biolegend |
103216 |
| Rat anti-mouse CD45 monoclonal antibody (30-F11) |
BD |
564279 |
| Hamster anti-mouse CD49a monoclonal antibody (Ha31/8) |
BD |
740144 |
| Rat anti-mouse CD49b monoclonal antibody (DX5) |
BioLegend |
108918 |
| Rat anti-mouse CD62L monoclonal antibody (MEL-14) |
BioLegend |
104433 |
| Mouse anti-mouse CD64 monoclonal antibody (X54-5/7.1) |
BioLegend |
139309 |
| Armenian hamster anti-mouse CD69monoclonal antibody (H1.2F3) |
BioLegend |
104530 |
| Rat anti-mouse CD8 monoclonal antibody (53-6.7) |
BD |
612898 |
| Rat anti-mouse CD88 monoclonal antibody (20/70) |
BD |
747227 |
| Rat anti-mouse CD90 monoclonal antibody (30-H12) |
BioLegend |
105329 |
| Rat anti-mouse CD90 monoclonal antibody (30-H12) |
BioLegend |
105327 |
| Mouse anti-mouse CX3CR1 monoclonal antibody (SA011F11) |
BioLegend |
149027 |
| Rat anti-mouse ESAM monoclonal antibody (1G8/ESAM) |
BioLegend |
136203 |
| Rat anti-mouse F4/80 monoclonal antibody (BM8) |
BioLegend |
123135 |
| Rat anti dog/cat/pig/mouse Foxp3 monoclonal antibody (FJK-16s) |
eBioscience |
61-5773-82 |
| Rat anti-mouse Ly6C monoclonal antibody (HK1.4) |
BioLegend |
128037 |
| Rat anti-mouse Ly6G monoclonal antibody (1A8) |
BD |
565707 |
| Rat anti-mouse/human MHCII monoclonal antibody (M5/114.15.2) |
BioLegend |
107616 |
| Rat anti-mouse/human MHCII monoclonal antibody (M5/114.15.2) |
BioLegend |
107622 |
| Mouse anti-mouse NK1.1 monoclonal antibody (PK136) |
Biolegend |
108724 |
| Mouse anti-mouse NK1.1(monoclonal antibody PK136) |
BioLegend |
108745 |
| Rat anti-mouse Siglec-F monoclonal antibody (E50-2440) |
BD |
562757 |
| Rat anti-mouse Siglec-F monoclonal antibody (E50-2440) |
Biolegend |
747316 |
| anti-mouse Siglec-H monoclonal ibody (551) |
oscience |
46-0333-80 |
| Hamster anti-mouse TCRb monoclonal antibody (H57-597) |
Biolegend |
109209 |
| Rat anti-mouse Tim4 monoclonal antibody (RMT4-54) |
Biolegend |
130007 |
| Mouse anti-mouse XCR1 monoclonal antibody (ZET) |
Biolegend |
148220 |
| Rat anti-mouse CD192 (CCR2)-PE |
BD Pharmingen |
747963 |
| Rat anti-mouse CD182 (CXCR2)-BV421 |
BD Pharmingen |
566622 |
| Recombinant human anti-mouse IL-1β pro-form antibody (REA577) |
Miltenyi Biotec |
130-109-043 |
| Recombinant human anti-mouse IL-6 antibody (REA1034) |
Miltenyi Biotec |
130-117-582 |
| Rat anti-mouse CD45 monoclonal antibody (30-F11) |
oscience |
13-0451-82 |
| Mouse anti-human CD16 monoclonal antibody (3G8) |
BD Pharmingen |
612944 |
| Mouse anti-human CD161 monoclonal antibody (HP-3G10) |
BD Pharmingen |
749223 |
| Mouse anti-human CD19 monoclonal antibody (HIB19) |
eBioscience |
62-0199-42 |
| Mouse anti-human CD45 monoclonal antibody (2D1) |
BioLegend |
368549 |
| Mouse anti-human CD3 monoclonal antibody (UCHT1) |
BD Pharmingen |
612895 |
| Mouse anti-human CD8a monoclonal antibody (RPA-T8) |
BioLegend |
301038 |
| Mouse anti-human CD4 monoclonal antibody (RPA-T4) |
BioLegend |
300519 |
| Mouse anti-human CD56 monoclonal antibody (5.1H11) |
BioLegend |
362543 |
| Mouse anti-human TCR Vα7.2 monoclonal antibody (3C10) |
BioLegend |
351705 |
| Mouse anti-human IL-17A monoclonal antibody (BL168) |
BioLegend |
512320 |
| Fc block (clone 2.4G2) |
Produced in house |
568093 |
| Rat anti-mouse NKG2D (MI-6) |
eBioscience |
16-5880-82 |
| goat anti-rat IgG-HRP |
Jackson ImmunoResearch |
112-035-003 |
| donkey anti-goat IgG-HRP |
Invitrogen |
A15999 |
| αSMA 1A4 |
Invitrogen |
14-9760-82
|
| αCD3 145-2C11 |
eBioscience |
16-0032-82 |
| Ki-67 (TEC-3) |
Dako |
M7240 |
| Goat anti-human IL-17A |
R&D |
AF-317-NA |
| αMICA/B (BAMOI) |
Made in house (AS) |
|
| αULBP1 (AUMO2) |
Made in house (AS) |
|
| αULBP3 (CUMO3) |
Made in house (AS) |
|
| goat anti-mouse IgG-HRP |
Jackson ImmunoResearch |
115-035-003 |
| Rabbit anti-mouse CD45 (D3F8Q) |
Cell Signaling |
70257S |
| Goat anti-mouse IgG Alexa Fluor-647 |
Abcam |
ab150083 |
| Rat anti-mouse Ki-67 |
Invitrogen |
14-5698-82 |
| Goat anti-rat IgG Alexa Fluor 594 |
Invitrogen |
1008647 |
| Capture antibody IL-17A |
eBioscience |
eBio17CK15A5 |
| biotinylated detection antibody |
eBioscience |
eBio17B7 |
| Polyvinyl alcohol 4-88 |
Fluka Chemika |
81381 |
| DAPI (4’,6-Diamino-2-Phenylindole, Dilactate) |
BioLegend |
422801 |
| mouse tetramer MR1 5-OP-RU-PE |
NIH Tetramer Facility |
|
| mNKG2D-hFc |
produced in house |
|
| Brefeldin A |
eBioscience |
00-4506-51 |
| TCRVγ6 (17D1) was made in house |
|
00-4506-51 |
| Citrate (tri-sodium citrate dehydrate) |
Kemika |
1405407 |
| Collagenase I |
Sigma Aldrich |
1148089 |
| Collagenase (IV) D |
Sigma Aldrich |
11088858001 |
| DMEM (4.5 g/L glucose, w L-glutamine) |
Pan Biotech |
P04-01549 |
| DMSO (Dimethyl sulphoxide) |
Gram-mol |
P04-03550 |
| DPBS (Dulbecco’s phosphate-buffered saline) |
Pan Biotech |
P120601 |
| Fetal Bovine Serum (pretested for ES cells) |
Pan Biotech |
P04-36500 |
| Fetal Bovine Serum (FBS Standard) |
Pan Biotech |
2602-P260328P30-2602 |
| Fixation/Permeabilization Concentrate |
Invitrogen |
P30-3306 |
| Fixation/Permeabilization Diluent |
Invitrogen |
00-5123 |
| Formaldehyde (4% NB) |
Biognost |
00-5223 |
| DNase |
|
FNB4 |
| HBSS |
Sigma Aldrich |
H8264 |
| HBSS w/o |
Sigma Aldrich |
H55037C |
| IC Fixation Buffer |
Invitrogen |
P04-80100 |
| ACK (Ammonium-Chloride-Potassium) |
Sigma Aldrich |
254134, 237205 |
| Lymphoprep |
Serumwerk Bernburg |
04-03-9391/01 |
| Ionomycin |
Sigma Aldrich |
407952 |
| HEPES |
Merck |
7365-45-9 |
| Mayer’s hematoxylin |
Sigma Aldrich |
MDL# MFCD00078111 |
| Streptavidin-PE-Cyanine5 |
eBioscience |
15-4317-82 |
| Streptavidin- Super Bright 436 |
eBioscience |
62-4317-82 |
| Streptavidin-Super Bright 645 |
eBioscience |
64-4317-82 |
| Streptavidin-APC-eFluor780 |
eBioscience |
47-4317-82 |
| ketamine |
Vetoquinol |
Vm 08007/4090 |
| xylazine |
Vetoquinol |
VTQ417633 |
| NucleoZOL |
Macherey-Nagel |
593-84-0 |
| o-Phenylenediamine dihydrochloride(OPD) |
Sigma Aldrich |
740404 |
| Penicillin-Streptomycin |
Pan Biotech |
P4664 |
| Percoll |
Cytiva |
P06-07100 |
| Permeabilization Buffer (10x) |
Invitrogen |
17089101 |
| EDTA VWR Life Sciences |
(VWR Life Sciences) |
00-8333 |
| PMA (Phorbol 12-myristate 13- acetate) |
Sigma Aldrich |
P1839 |
| RBC Lysis Buffer |
eBioscience |
00-4333-57 |
| Liberase™ Research Grade |
Sigma-Aldrich |
00430054 |
| Williams E medium PAN Biotech |
PAN Biotech |
P04-29500 |
| Oleic acid |
Merck |
05508 |
| RPMI 1640 |
Pan Biotech |
P04-18047 |
| Sirius Red (Direct Red 80) |
Sigma Aldrich |
P04-22100 |
| Cholesterol |
SSNIFF |
R143C040 |
| Sodium Pyruvate |
Sigma Aldrich |
P2256 |
| Sunflower Seed Oil |
Sigma Aldrich |
47123 |
| Topro3 |
Invitrogen |
S5007 |
| Trypsine/EDTA (10x) |
Pan Biotech |
T3605 |
| Tween20 |
Sigma Aldrich |
P10-024100 |
| IL-17A |
PreproTech |
P1379 |
| Fructose |
SSNIFF |
R111F040 |
| Fructose |
SpecialIngredients |
5056191008429 |
| MCD (methionine and choline deficient diet) |
SSNIFF |
E15653-94 |
| Albumin fraction V |
Roth |
T844.3 |
| NaN3
|
Alfa Aesar |
U17D012 |
| Fixable Viability Dye eFluor 780 |
eBioscience |
65-0865-14 |
| Fixable Viability Dye eFluor 506 |
eBioscience |
65-0866-14 |
| Bodipy493/503
|
BD Biosciences |
D3922 |
| Fixable Viability Kit (LIVE/DEAD Blue) |
Thermo fisher |
L23105 |
| EDTA |
Kemika |
1136808 |
| Tris buffer |
Roth |
4855.2 |
| goat serum |
Dako |
X090710-8 |
| H2O2
|
Gram mol |
P154903 |
| DAB |
Dako |
K3468 |
| Entellan |
Sigma-Aldrich, USA |
1079600500 |
| Instant Eosin |
Thermo Scientific |
6765540 |
| TritonX |
Sigma Aldrich |
X100 |
| SYBR Green dye |
Eurogentec |
UF-NSMT-B0701 |
| Glacial acetic acid |
Kemika |
1500301 |
| Picric acid |
Sigma Aldrich |
319287 |
| Reverse Transcription Core Kit |
Eurogentec |
RT-RTCK-03 |
| Takyon No Rox SYBR MasterMix dTTP Blue |
Eurogentec |
43-695 |
| BrdU flow kit |
BD Biosciences |
559619 |
| FoxP3 staining buffer set |
eBioscience |
00-5523-00 |
| Fix/Perm kit |
BD Biosciences |
554714 |
| Balbc |
Jackson Laboratories |
JAX:000651 |
| C57BL/6 (B6; line 664) |
Jackson Laboratories |
JAX: 000664 |
| C57BL/6J CD45.1 (2014) |
Jackson Laboratories |
JAX: 002014 |
|
LysMCre (4781) |
Jackson Laboratories |
JAX: 004781 |
|
AlbCre (3574) |
Jackson Laboratories |
JAX: 003572 |
| GFAP-Cre line 77.6 (024098) |
Jackson Laboratories |
JAX: 024098 |
|
Cαfl/fl
|
Produced in house |
PMID: 11447257 |
| TCRd-/-
|
Prof. AdrianHayday; London, England |
JAX: 002116 |
|
Klrkl-/- |
Produced in house |
PMID: 19631564 |
|
Klrklfl/fl
|
Produced in house |
PMID: 21898152 |
|
lll7rafl/fl
|
Ari Waisman;Mainz, Germany |
PMID: 22951726 |
| DelCre
|
Taconic |
JAX: 0012524 |
| Mr1–/– Rorcgt-GFPTG B6-MAITCAST |
Olivier Lantz; Paris,France |
PMID: 6524590 |
| Mr1+/+ Rorcgt-GFPTG B6-MAITCAST |
Olivier Lantz; Paris,France |
PMID: 6524590 |
|
NCR1Cre
|
Veronica Sexl;Vienna, Austria |
PMID: 21127177 |
|
Cd1d-/-
|
Thomas Wunderlich, Max Planck, Germany |
JAX: 008881 |
|
Cd4Cre |
D. Littman; New York, NY, USA |
PMID: 12121668 |
|
Hcst-/- |
M. Colonna; St.Louis, MO, USA |
PMID: 19247984 |
| HPRT forward 5’- TGAAGAGCTACTGTAATGATCAGTCAAC-3’ |
Metabion International AG |
Custom |
| HPRT reverse 5’-AGCAAGCTTGCAACCTTAACCA-3’) |
Metabion International AG |
Custom |
| (PanRae1ε forward5’-CAGGTGACCCAGGGAAGATG-3’; |
Metabion International AG |
Custom |
| PanRae1ε reverse 5’-CTCAACTCCTGGCACAAATCG-3’):, |
Metabion International AG |
Custom |
| MULT1 (MULT1 forward 5’-CTCATAGGAACAGCATGA-3’ |
Metabion International AG |
Custom |
| MULT1 reverse 5’-TCCTGTGAAATGTTTGTC-3’), |
Metabion International AG |
Custom |
| H60 (H60 forward 5’-GAGCCACCAGCAAGAGCAA-3’ |
Metabion International AG |
Custom |
| H60 reverse 5’-CCAGTATGGTCCCCAGATAGCT-3’), |
Metabion International AG |
Custom |
| CXCL2 (CXCL2 forward 5’- CCCAGACAGAAGTCATAGCCAC-3’ |
Metabion International AG |
Custom |
| CXCL2 reverse 5’- CTTCCGTTGAGGGACAGCAG-3’), |
Metabion International AG |
Custom |
| CXCL1 (CXCL1 forward 5’- CAGACCATGGCTGGGATTCA-3’; |
Metabion International AG |
Custom |
| CXCL1 reverse 5’-ACTTGGGGACACCTTTTAGCAT-3’), |
Metabion International AG |
Custom |
| CCL2 (CCL2 forward 5’-GCCTGCTGTTCACAGTTGC-3’; |
Metabion International AG |
Custom |
| CCL2 reverse 5’- TTGAGCTTGGTGACAAAAACTAC-3’) |
Metabion International AG |
Custom |
| CXCL10 (CXCL10 forward 5’-ATGACGGGCCAGTGAGAATG-3’; |
Metabion International AG |
Custom |
| CXCL10 reverse 5’-AGGAGCCCTTTTAGACCTTTTT-3’) |
Metabion International AG |
Custom |
| 7500 Software |
Applied Biosystems; Life Technologies |
|
| Fiji/ImageJ |
Nature Methods,9(7), 676−682. |
doi:10.1038/nmeth.2019
|
| FlowJo |
FlowJo LLC, BD Life Sciences |
https://imagej.net/software/fiji/
|
| GraphPad Prism |
GraphPad Software |
|
| MicroWin |
Berthold Technologies |
|
| Cell^ B Soft Imaging System |
Olympus |
|
| Mithras LB940 ELISA plate reader |
Berthold technologies |
|
