Extended Data Fig. 4 |. COQ3 requires Mg²⁺for substrate coordination.

a. Unfolding temperature of COQ3 in the presence of a range of divalent cations (150 μM) or EDTA (1 mM). Individual data points corresponding to n = 2 independent measurements are shown. The bars of the histogram show the mean value of independent replicates. b. Microscale thermophoresis binding-check of NHS labelled COQ3 with 2 after pre-incubation with EDTA (1 mM). The substrate (2) does not cause any large difference in the normalised thermophoretic signal (the experiment without EDTA is shown in Fig. 2h). Individual data points corresponding to n = 3 independent measurements are shown. The error bars correspond to the standard deviations in n = 3 independent measurements for each datum.