Extended Data Fig. 1 |. Ancestral COQ proteins show good purity and no aggregation in gel-filtration.

Analytical (COQ3, COQ8B) or preparative (COQ4, COQ5, COQ6, COQ7, COQ9) Size Exclusion Chromatography profiles and SDS-PAGE analysis of purified COQ proteins (Supplementary Fig. 16). Size Exclusion Chromatography and SDS-PAGE analyses were repeated in n = 3 independent experiments for each purified COQ protein. Dashed lines indicate where lanes have been moved and rearranged in the gel to ease visual comparison; original gels can be found in Supplementary Fig. 16.