Fig. 5.

DN ESCRT mutants have no effect on the production of intracellular virus particles. Huh7 cells were electroporated with JFH-1/ΔE1E2 RNA and subsequently transfected with pcDNA-E1-p7(J4) and plasmids encoding a panel of wild-type and DN ESCRT proteins. Intracellular contents were liberated by repeated freeze–thaw cycles and infectious particles were quantified by focus-forming assay (a). Data were normalized to the titre produced in the presence of GFP only (100%). Lysates from the producer cells were analysed by Western blotting with the indicated antibodies (b). All error bars indicate SEM.