Fig. 6.

Inhibition of Vps4 and ESCRT-III blocks release of virus particles from Huh7 cells infected with full-length HCV. Huh7 cells were infected with Jc1 at an m.o.i. of 0.5 and subsequently transfected with plasmids encoding a panel of WT and DN ESCRT proteins. Infectious extracellular and intracellular particles were quantified by focus-forming assay (a, b). Extracellular results were normalized to titres produced in the presence of GFP only (100%). Lysates from the producer cells were analysed by Western blotting with the indicated antibodies (c). All error bars indicate SEM.