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. Author manuscript; available in PMC: 2024 Mar 4.
Published in final edited form as: J Gen Virol. 2009 Mar 4;90(Pt 6):1329–1334. doi: 10.1099/vir.0.009332-0

Fig. 2. Characterization of virus assembly and release of domain III mutants.

Fig. 2

(a) Huh7 cells were electroporated with the indicated virus RNAs and virus release into the culture supernatant was measured by a focus-forming assay. (b) Intracellular virus titres were measured by a focus-forming assay following cell disruption by repetitive freeze–thaw at 72 h p.t. (c, d) Extracellular (c) and intracellular (d) genomic RNA levels were measured at 120 h p.t. by quantitative RT-PCR. Data from three independent experiments are shown; bars indicate SEM. GND represents a corresponding polymerase-defective construct.