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. Author manuscript; available in PMC: 2024 Mar 4.
Published in final edited form as: J Gen Virol. 2009 Mar 4;90(Pt 6):1329–1334. doi: 10.1099/vir.0.009332-0

Fig. 3. Viral protein expression in infected cells.

Fig. 3

(a) Huh7 cells were electroporated with the indicated virus RNAs and harvested at 48 h p.t. by lysis in Glasgow lysis buffer (Harris & Coates, 1993). Protein (10 μg) was analysed by Western blotting with antisera to Core, NS2, NS5A and GAPDH as indicated. (b) Immunofluorescence analysis of NS5A (green) and Core (red) in infected cells at 72 h p.t. Representative images are shown.