Extended Data Fig. 4 (Related to Figure 4). AtEH1 recruits proteins with prion-like domains.

a, Plot of Prion-like proteins identified in the AtEH1 and TPLATE TurboID datasets. b, (Left) Plot of top 100 enriched proteins based on disorder content (MobiDB), $\overline{x}$ (mean) disorder score is indicated. (Right) plot showing abundance and disorder content of selected endocytic proteins. c, Partitioning assay of selected nuclear proteins with prion-like domains identified in the AtEH1-TurboID dataset. UBQ10:AtEH1_FL-GFP was co-expressed with mScarlet tagged client proteins in N. benthamiana epidermal cells. Partitioning was observed in the cytosol (Med15a, Med25) and in the nucleus (TAF12b). Scale bars = 5 μm (c). For c the experiment was performed two times with similar results. Source data are provided as a Source Data file.