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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Nat Cell Biol. 2024 Feb 12:10.1038/s41556-024-01354-6. doi: 10.1038/s41556-024-01354-6

Figure 5. Condensation of AtEH1 facilitates clathrin recruitment and assembly.

Figure 5

a, Fluorescence and electron microscopy images of hypocotyl resin sections from A. thaliana seedlings over-expressing AtEH1 (35S:AtEH1-GFP). Yellow arrowheads indicate chloroplasts used as natural landmarks for correlation. b, Electron tomography (ET) reconstructions of the selected regions from a. Clathrin-like lattices and triskelia (orange arrowheads), and electron dense accumulations (yellow arrowheads) are formed within AtEH1 condensates. Inset shows a clathrin-coated vesicle (CCV) nearby the condensate. c, Representative airyscan images of clathrin light chain 2 (CLC-mKo) and AtEH1 (35S:AtEH1-GFP) in A. thaliana hypocotyl cells. Plot profiles show normalised fluorescence intensities from the indicated dashed lines. Clathrin is observed within and surrounding the condensates. Scale bars = as indicated (a), 50 nm (b), 2 μm (c). See also Extended Data Fig. 5; Video S2. Source data are provided as a Source Data file.