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. Author manuscript; available in PMC: 2024 Mar 23.
Published in final edited form as: Biotechnol Bioeng. 2023 Dec 7;121(3):959–970. doi: 10.1002/bit.28619

Figure 6. Gene expression analyses in C. elegans.

Figure 6

(a) Micrographic image of C. elegans on Day 0 of treatment. (b) Relative fold change of lys-2 and ilys-3 gene expression estimated via qPCR using the 2–ΔΔCt method and compared with the untreated control, with snb-1 as the reference gene for normalization. The BG:OP50 ratios tested were 1:1 and 2:1 where, 1:1 is 1 × 104:1 × 104 units/plate, and 2:1 is 2 × 104:1 × 104 units/plate of BG:OP50; BG1 = 1 × 104 units/plate and BG2 = 2 × 104 units/plate. *p < 0.05, **p < 0.005, ***p < 0.001 and ns: not significant. BG, bacterial ghost.