REAGENT or RESOURCE		SOURCE	IDENTIFIER
Antibodies
Rabbit anti-MAP2		Abcam	Ab32454; RRID: AB_776174
Rabbit anti-VGAT		Synaptic Systems	131013; RRID: AB_2189938
Rabbit anti-Phospho-CREB		Cell Signaling	9198; RRID: AB_2561044
Rabbit anti-CREB		Cell Signaling	9197S; RRID: AB_331277
Rabbit anti-GAPDH		Cell Signaling	2118S; RRID: AB_561053
Rabbit anti-H3K4me3		Abcam	Ab32356; RRID: AB_732924
Rabbit anti-GABA		Sigma-Aldrich	A0310; RRID: AB_476667
Mouse anti-SET1A		Santa Cruz	SC-515590; RRID: N/A
Mouse anti-γ-tubulin		Sigma-Aldrich	T5326; RRID: AB_532292
Mouse anti-Gephyrin		Synaptic Systems	147011; RRID: AB_887717
Guinea pig anti-Synapsin 1/2		Synaptic Systems	106004; RRID: AB_1106784
Goat anti-rabbit IgG		Invitrogen	G-21234; RRID: AB_2536530
Goat anti-mouse IgG		Jackson Immuno Research Laboratories	115-035-062; RRID: AB_2338504
Goat-anti rabbit Alexa 568		Invitrogen	A11036; RRID: AB_10563566
Goat-anti-mouse Alexa 488		Invitrogen	A11029; RRID: AB_2534088
Goat anti-guinea pig Alexa Fluor 568		Invitrogen	A11075; RRID: AB_2534119
Goat anti-guinea pig Alexa Fluor 647		Invitrogen	A21450; RRID: AB_141882
Bacterial and virus strains
AAV2-hSyn-mCherry		UNC Vector Core	AV5033E
Chemicals, peptides, and recombinant proteins
Accutase		GIBCO	A11105-01
B27 Supplement		GIBCO	0080085SA
Cytosine β-D-arabinofuranoside (Ara-C)		Sigma-Aldrich	C1768-100MG
BDNF, human recombinant		Promokine	C66212
DAKO fluorescent mounting medium		DAKO	S3023
DMEM/F12		GIBCO	11320–074
Doxycycline		Sigma-Aldrich	D9891-5G
DPBS		GIBCO	14190–094
E8 Basal Medium		GIBCO	A1517001
Fetal Bovine Serum (FBS)		Sigma-Aldrich	F2442-500ML
Fluo-8-AM		Abcam	Ab142773
Forskolin		Sigma-Aldrich	F6886
G418		Sigma-Aldrich	4727878001
GlutaMAX		GIBCO	35050061
Hoechst 33342		Thermo-Fisher	H3570 N/A
H89		Tocris	2910
KT5720		Tocris	1283
Matrigel		Corning	356237
Non-essential amino acid solution NEAA		Sigma-Aldrich	M7145
N2		GIBCO	17502–048
Neurobasal Medium		GIBCO	21103–049
NT-3, human recombinant		Promokine	C66425
ORY1001	SelleckChem			RG6016
Penicillin/Streptomycin	Sigma-Aldrich			P4333
Poly-L-ornithine hydrobromide (PLO)	Sigma-Aldrich			P3655-10MG
Primocin	Invivogen			ANT-PM-05
Puromycin	Invivogen			ANT-PR-1
Picrotoxin	Tocris			1128
Recombinant Human Laminin LN521	Biolamina			2021–21
RevitaCell	Thermo-Fisher			A2644501
ReLeSR	Stem Cell Technologies			058072
SQ22536	Sigma-Aldrich			S153
Triton X-100	Sigma-Aldrich			9002-93-1
Critical commercial assays
24-well MEA system	Multichannel Systems, MCS GmbH, Reutlingen, Germany			N/A
Zeiss Axio Imager Z1 Olympus	Zeiss			https://www.zeiss.com/
Multiclamp 700B amplifier	Molecular Devices, Wokingham, United Kingdom			https://www.moleculardevices.com/
cAMP ELISA kit	Abcam			Ab133051
BCA protein assay kit	Thermo-Fisher			23225
iMark™ Microplate Absorbance Reader	Bio-Rad			N/A
Deposited data
RNA-seq	This paper			GEO: GSE180648
Code	This paper			Zenodo: https://doi.org/10.5281/zenodo.6409947
Experimental models: Human iPSC lines
CTR-WTC	Corielll Institute			https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM25256&Product=CC
WTC-CRISPR-clone 1	Generated in this study			N/A
WTC-CRISPR-clone 2	Generated in this study			N/A
CTR-WTC-Ngn2/rtTA	Generated in this study			N/A
CTR-WTC-Ascl1/rtTA	Generated in this study			N/A
WTC-CRISPR-clone 1- Ngn2/rtTA	Generated in this study			N/A
WTC-CRISPR-clone 1- Ascl1/rtTA	Generated in this study			N/A
WTC-CRISPR-clone 2- Ngn2/rtTA	Generated in this study			N/A
WTC-CRISPR-clone 2- Ascl1/rtTA	Generated in this study			N/A
CTR-409b2	Yamanaka, Shinya / RIKEN BRC Generated			HPS0076; 409B2; RRID: CVCL_K092
409b2-CRISPR	Generated in this study			N/A
CTR-409b2- Ngn2/rtTA	Generated in this study			N/A
CTR-409b2- Ascl1/rtTA	Generated in this study			N/A
409b2-CRISPR- Ngn2/rtTA	Generated in this study			N/A
409b2-CRISPR- Ascl1/rtTA	Generated in this study			N/A
Experimental models: Organisms/strains
Wistar WT Rat (Dissociated astrocytes)	Charles River			N/A
Recombinant DNA
Lentivirus psPAX2 packaging vector Lentivirus	psPAX2 was a gift from Didier Trono			RRID: Addgene_12260; http://addgene.org/12260
Lentivirus VSVG envelope glycoprotein vector pMD2-G	pMD2.G was a gift from Didier Trono			RRID: Addgene_12259; http://addgene.org/12259
pSpCas9(BB)-2A-Puro (PX459)		Addgene		62988
pLVX-EF1α-(Tet-On-Advanced)-IRES-G418(R) lentiviral vector		Mossink et al., 2021		N/A
pLVX-(TRE-thight)-(MOUSE) Ngn2-PGK-Puromycin(R) lentiviral vector		Mossink et al., 2021		N/A
pLV[TetOn]-Puro-TRE > mAscl1 lentiviral vector		Mossink et al., 2021		N/A
Software and algorithms
GraphPad Prism		GraphPad software		https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798
Fiji		National Institutes of Health		https://imagej.nih.gov/ij/download.html RRID: SCR_003070
ClampfitV 10.2		Molecular Devices, LLC., CA, USA		RRID: SCR_011323
MATLAB 2014b		Mathworks		RRID: SCR_001622
Multiwell Analyzer		Multichannel Systems, MCS GmbH, Reutlingen, Germany		N/A
Neurolucida 360		MBF-Bioscience, Williston, ND, USA		RRID: SCR_016788
Image lab		Bio-Rad		https://www.bio-rad.com/en-nl/product/image-lab-software/