Fig. 3. Miz1 is required for IgG1⁺ GC B cell survival during positive selection.

(A) Schematic of Myc^neg LZ, Myc^pos LZ, Myc^pos DZ, Myc^neg DZ four consecutive GC B cell populations, and processes involved during positive selection.

(B) Gating strategy for the four consecutive GC B cell populations as in (A).

(C) Percentages of EdU⁺ cells within each of the four consecutive GC B cell populations in both eYFP and MIZ1^KO mice.

(D) Percentages of active-Caspase3⁺ cells within each of the four consecutive GC B cell populations.

Each symbol (C: eYFP n = 6, MIZ1^KO n = 6; D: eYFP n = 4, MIZ1^KO n = 4) represents an individual mouse; small horizontal lines show mean and SEM. Data in (C, D) is representative from two experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; Two-way ANOVA in (C, D)). ns, not significant.