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. Author manuscript; available in PMC: 2024 May 27.
Published in final edited form as: J Control Release. 2022 Jan 24;343:131–141. doi: 10.1016/j.jconrel.2022.01.030

Figure 2. Sequential Phagocytosis among Immune Cells from Humans, Drosophila and in Mice.

Figure 2

A − Uptake of 500 nm-carboxylated polystyrene (PS) particles by different types of phagocytic immune cells studied using the following cell to particle ratio and incubation times (t1+t2): dHL-60 − 1:100 for 2+2 hours; monocytes − 1:200 for 18+4 hours; neutrophils 1:200 for 4+4 hours. Uptake percentages were quantified using flow cytometry. B − Ex vivo uptake among hemocytes isolated from Drosophila larvae incubated for 2 hours with 3 μm BSA-TRITC-adsorbed PS particles at a cell to particle ratio of 1:20, followed by incubation with 500 nm-carboxylated PS particles for 2 hours at a cell to particle ratio of 1:200. C − Measurement of uptake by mouse peritoneal macrophages (F4/80+ cells) following sequential intraperitoneal injection of 6 × 106 500 nm-carboxylated polystyrene (PS) particles, labelled with different fluorophores, in BALB/c (n=6) or C57BL6 (n=2) mice. Uptake was quantified using flow cytometry. In all the data sets, “By” indicates uptake by bystander cells and “Seq” represents sequential uptake. Data sets are representative of n ≥ 3. * = p < 0.05; ** = p < 0.01; and *** = p < 0.001 calculated using Student’s t-test.