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. Author manuscript; available in PMC: 2024 May 27.
Published in final edited form as: J Leukoc Biol. 2022 Feb 16;112(3):395–409. doi: 10.1002/JLB.1A0321-132RR

Figure 5. Tracking labelled neutrophils.

Figure 5

A - Gating strategy used to determine labelled (EdU positive) neutrophils. Fluorescence-minus-one (FMO) plots were used to ascertain gate positions. Plots are representative of multiple independent experiments. B – D – Labelled neutrophil percentages in the bone marrow (B) blood (C) and the peritoneal cavity (D) at different times. N = 3-7 mice/time point/per group pooled from at least 3 independent experiments including both male and female mice (no difference was observed based on sex of mice). For statistical analyses, at each time point, a one-way ANOVA was performed followed by Tukey post-test. *, $ and # indicates comparison between mock vs chitosan, alginate vs chitosan and mock vs alginate, respectively. * = p < 0.05; ** = p < 0.01; *** = p < 0.001; and **** = p < 0.0001