Figure 5. rDC require MyD88 in order to become fully activated in response to IAV antigen acquisition.

(A) Schematic of experimental approach taken in (B-G) (B) Percent of peripheral CD11b+ cells which were CD45.1⁺ or CD45.2⁺ at 6 weeks post bone marrow transfer (n=5, 3 experiments) (C-D) Percent of cDC in the lung (C) and dLN (D) positive for ZsGreen was assessed by flow cytometry amongst MyD88^-/- and wildtype cDC (n=10, 3 experiments) (E-G) Differences in CD80 and CD86 were quantified upon acquisition of viral derived ZsGreen in both wildtype and MyD88^-/- cDC within the lung (J), and within the mDC (K) or rDC (L) in the dLN (n=18, 3 experiments). Statistical differences were determined by student T test (E-G) and one-way ANOVA with Tukey post-test (C, D). P values: ns P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001. Data are shown as mean ± SEM