Table 1. Data Collection and refinement statistics, related to Figure 1.
| Data collection and processing | 60S subunit | 40S subunit |
|---|---|---|
| Microscope | Titan Krios | Titan Krios |
| Voltage | 300 kV | 300 kV |
| Magnification | 75,000x | 75,000x |
| Detector | Falcon III | Falcon III |
| Sampling (Å/pixel) | 1.07 | 1.07 |
| Total electron dose (e-/Å2) | 44.6 | 44.6 |
| Symmetry imposed | C1 | C1 |
| Resolution (Å) by Gold-standard FSC0.143 | 2.7 | 2.9 |
| Map Sharpening B-factors (Å2) | -121 | -100 |
| Atomic model composition | ||
| Chains RNA/Protein | 3/44 | 1/38 |
| Non-hydrogen atoms | 116711 | 68750 |
| Amino acids | 6191 | 4411 |
| Nucleotides | 3134 | 1571 |
| Number of ligand atoms | 317 | 87 |
| Zn2+/Mg2+/K+ | 3/242/72 | 1/70/16 |
| Refinement statistics | ||
| Model to map CC (CCmask/CCbox/CCpeaks/CCvolume) | 0.85/0.75/0.77/0.84 | 0.81/0.78/0.72/0.82 |
| Resolution (Å) by model-to-map FSC0.5 (masked/unmasked) | 2.7/2.8 | 3.1/3.3 |
| Bond length RMSD (Å) | 0.003 | 0.003 |
| Bond angle RMSD (°) | 0.634 | 0.707 |
| B-factors for protein residues (min/max/mean) | 0.00/51.77/6.88 | 22.62/278.33/123.17 |
| B-factors for RNA residues (min/max/mean) | 0.00/61.06/5.71 | 17.89/308.52/107.12 |
| B-factors for ligand (min/max/mean) | 0.00/53.41/11.45 | 15.42/247.0/102.09 |
| Validation | ||
| Clash score | 6.67 | 14.84 |
| MolProbity score | 2.14 | 2.63 |
| Ramachandran plot (%) (Favoured/allowed/outliers) | 95.61/4.39/0 | 91.81/8.12/0.07 |
| Rotamer outliers (%) | 4.1 | 3.99 |
| CaBLAM outliers (%) | 2.36 | 4.01 |
*
As data collection statistics for subunits of 80S (EMD – 36334 and EMD – 36333 is same as the data collection statistics of 40S as these maps were obtained from the same dataset by focus refinement.