REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Experimental model; Tissue
Wheat germ	Bob’s RedMill	B00AWNXMI2
Software and algorithms
RELION 3.1	Scheres, S.H.W.201258	https://relion.readthedocs.io/en/release-3.1/index.html
MotionCor2 v1.2.1	Zivanov et al., 201956	https://emcore.ucsf.edu/ucsf-software
CTFFIND v4.1.13	Rohou and Grigorieff, 201557	https://grigoriefflab.umassmed.edu/ctffind4
Cryosparc v3.32	Punjani et al., 201760	https://cryosparc.com
Chimera v1.12	Petterson et al., 200472	https://www.cgl.ucsf.edu/chimera/
ChimeraX v1.3	Petterson et al., 202173	https://www.cgl.ucsf.edu/chimerax/
Coot v0.9.4.1	Emsley et al., 200466	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Phenix v1.20	Adams et al., 201062	https://phenix-online.org/
CCPEM v1.6.0		https://www.ccpem.ac.uk/
Deposited Data
Map for Cryo-EM structure of 80S ribosomal subunit from wheat germ	This Study	EMD-36334
Map for Cryo-EM structure of 60S ribosomal subunit from wheat germ	This Study	EMD-36331
Map for Cryo-EM structure of 40S ribosomal subunit from wheat germ	This Study	EMD-36332
Map for Cryo-EM structure of the body of 40S ribosomal subunit from wheat germ	This Study	EMD-36333
Coordinates for cryo-EM structure of 40S ribosomal subunit from wheat germ	This Study	PDB:8JIW
Coordinates for cryo-EM structure of 60S ribosomal subunit from wheat germ	This Study	PDB:8JIV
Cryo-EM structure of pre-60S ribosomal subunit	Wu S et al., 201649	PDB: 3JCT
Structure of 80S ribosome from wheat germ at 5.5Å resolution	Armache et al., 201464	PDB: 4V7E
High resolution structure of 60S ribosome from tomato	Cottilli et al., 202216	PDB: 7QIZ
High resolution structure of human 80S ribosome	Natchiar et al., 201714	PDB: 6QZP
High-resolution structure of ribosome from thermophilic fungi	Kisionaite et al., 202245	PDB: 7OLC
High resolution structure of ribosome from Leishmania	Shalev-Benami et al., 201767	PDB: 6AZ3
High resolution structure of ribosome from Euglena	Matzov et al., 202068	PDB: 6JZ3
High resolution structure of ribosome from yeast	Ben-Shem et al., 201170	PDB: 4V88
Reagents
HEPES	SRL	Cat#63732
Potassium Chloride	SRL	Cat#38630
Magnesium acetate	Sigma	Cat#228648
Calcium chloride	Sigma	Cat#C1016
Dithiothreitol	SRL	Cat#17315
Heparin	SRL	Cat#58389
Benzamidine	Sigma	Cat#B-6506
Soybean Trypsin Inhibitor	Sigma	Cat#T6522
Phenylmethylsulphonyl fluoride	Roche	Cat#39212622
Ethylenediaminetetraacetic acid	Sigma	Cat#60004
β-Mercaptoethanol	Sigma	Cat#444203
Tris-HCl	Sigma	Cat#10812846001
Puromycin	Sigma	Cat#P8833