Skip to main content
. Author manuscript; available in PMC: 2024 Jul 3.
Published in final edited form as: J Neurosci. 2024 May 1:e2148232024. doi: 10.1523/JNEUROSCI.2148-23.2024

Figure 5. FUS expression does not alter axonal RNA transport dynamics.

Figure 5

a) Sample preparation procedure. mRNA is co-injected with Cy5-UTP, which becomes incorporated into all embryonically synthesised RNA. RGCs are cultured as previously. Cy5-labelled RNA is visible as granules in the axon and growth cone (Piper et al., 2015; Wong et al., 2017). b) Sample image of RNA granules in GFP-expressing RGC axon (scale bar: 5 μm). c) Sample kymograph and track detection by KymoButler (Jakobs et al., 2019). d) Average granule density per unit length for individual axons is not altered by mutant FUS expression. (Total number of axons analysed: nGFP = 180; nFUS(WT) = 138; nFUS(P525L) = 155; nFUS(16R) = 136.) e) Average frame-to-frame displacement for individual granules in 60 s is not altered by mutant FUS expression.(Total number of tracks identified: nGFP = 8587; nFUS(WT) = 6710; nFUS(P525L) = 7609; nFUS(16R) = 6202) d-e) N=3 replicates for each condition. Kruskal-Wallis tests with Bonferroni correction, error bars indicate standard errors in means.