Table 3. Pharmacological evaluation analogues 12a-20 tested at 10 μM against an ACh-mediated concentration-response curve.

Compound #	A	Δbaselinea	ΔpEC50	ΔEmaxb
1e		103.2 ± 2.85	1.48 ± 0.20	44.7 ± 2.74
12a		13.0 ± 2.18*	0.88 ± 0.07*	41.6 ± 2.67
12b		6.49 ± 1.85*	0.63 ± 0.07*	25.6 ± 2.78*
12c		47.2 ± 2.93*	0.17 ± 0.13*	56.8 ± 4.81
12d		90.6 ± 7.00*	1.49 ± 0.28	66.6 ± 5.99*
12e		48.5 ± 2.91*	−0.34 ± 0.16*	35.1 ± 5.31
16		20.0 ± 4.81*	1.07 ± 0.20	11.8 ± 6.21*
20		24.5 ± 2.44*	1.06 ± 0.10	13.9 ± 2.95*

Data represent the mean ± SEM of 4 independent experiments performed in duplicate. Only the change in pEC₅₀, baseline and E_max for the 10 μM concentrations of PAMs are reported, with the full ACh concentration-response curves in the presence of 10 μM PAMs shown in Supp. Fig. 4. Data were analysed by one-way ANOVA and compared to the control PAM, 1e, with a post-hoc Dunnett test,

where *p < 0.05 was considered to be significantly different.

^aΔbaseline is expressed as a percentage of the baseline ACh response in the absence of PAM.

^bΔE_max is expressed as a percentage of the maximum ACh response in the absence of PAM.