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. Author manuscript; available in PMC: 2024 Aug 23.
Published in final edited form as: Nat Protoc. 2022 Apr 27;17(7):1579–1620. doi: 10.1038/s41596-022-00691-w

Fig. 5. Inducing and checking expression of all-optical constructs.

Fig. 5

a, Strategies for achieving co-expression of all-optical constructs. b, Co-expression of all-optical constructs in superficial primary somatosensory and visual cortices (L2/3 S1 and V1). Left, experimental prep schematic; a chronic imaging window is installed on the cortical surface with either dual adeno-associated virus (AAV) expression of GCaMP and C1V1 (S1) or AAV expression of C1V1 in GCaMP transgenic mice (V1). Two right-hand images, example of healthy co-expression. c, Co-expression of all-optical constructs in deep cortex (L5 V1). Left, experimental preparation schematic; a chronic imaging window is installed on the cortical surface of L5-Cre transgenic mice injected with FLEX-C1V1 and FLEX-GCaMP. Right, example of healthy co-expression. d, Co-expression of all-optical constructs in subcortical structures (hippocampal CA1 and CA3). Left, experimental preparation schematic; cortical aspiration combined with a canula + chronic imaging window in CA1/CA3-Cre transgenic mice injected with FLEX-C1V1 and FLEX-GCaMP. Two right-hand images, example of healthy co-expression. AP, antero-posterior; bregma, junction point of the coronal and sagittal sutures of the skull; DV, dorso-ventral; lambda, junction point of the lambdoid and sagittal sutures of the skull; ML, medio-lateral.