Skip to main content
. Author manuscript; available in PMC: 2024 Sep 10.
Published in final edited form as: Sci Adv. 2021 May 7;7(19):eabf0466. doi: 10.1126/sciadv.abf0466

Fig. 6. MiR-210 deletion in macrophages counters inflammatory pathways and activation markers in response to endotoxin.

Fig. 6

(A and B) Top 10 GO terms enriched, based on gene ratio, for significantly downregulated (A) and upregulated (B) genes in miR-210 KO versus WT BMDMs after 42-hour LPS/IFN-γ stimulation. Colour of the dots indicates adjusted P value. (C and D) MFI of MHC-II (C) and iNOS (D) of WT and miR-210 KO BMDMs, untreated or after 24-hour LPS/IFN-γ. (E) IL-6 concentration (MSD) in the medium of WT and miR-210 KO BMDMs, untreated or after 12-hour LPS/IFN-γ. (F) Fold changes of IL-6 (ELISA) in the medium of WT and miR-210 KO BMDMs after 24-hour LPS/IFN-γ. BMDMs were electroporated 48 hours before LPS/IFN-γ treatment with 2 siRNAs against ISCU or a control sequence. (G) MFI of MHC-II of WT and miR-210 KO BMDMs after 24-hour LPS/IFN-γ. Macrophages were transduced with a shRNA against ISCU or a control sequence. (H) MFI of MHC-II in liver macrophages (CD45+, CD11b+, Ly6G-, F4/80+) from Control (n=9) and miR-210 ΔM0 (n=9) mice. (I) MFI of MHC-II in spleen macrophages (CD45+, CD11b+, Ly6G-, MHC-II+) from Control (n=9) and miR-210 ΔM0 (n=10) mice. (J and K) IL-6 and IL-10 concentration (ELISA) in the plasma of Control (n=9-13) and miR-210 ΔM0 (n=10-11) mice. In vivo analysis was performed 18 hours after sublethal LPS i.p. injection (H-K). Data show a representative of three (C and D) or a pool of two (E, H to K), three (F) or five (G) independent experiments. P value was assessed by two-way ANOVA with Sidak’s multiple comparisons test (C-G) and two-tailed, unpaired, Student’s t-test (H-K). Statistical analysis: *p< 0.05; **p< 0.01; ***p< 0.001; graphs show mean ± SEM.