Fig. 4.

Antibody responses after Ad prime and OMV boost. Groups of 5 NIH mice were immunised with 10⁹ IU of Ad-P1.7,16 or 2.5 μg of OMVs (44/76 FetA_on PorA_on) according to the figure legend (A). Immunisation times are marked with arrows. Antibody responses were measured by ELISA against recombinant PorA rP1.7,16. Mean values with SD are displayed. Asterisks denote two-way ANOVA comparisons with Bonferroni multiple testing between groups Ad-P1.7,16-prime, OMV-boost and Ad-P1.7,16 alone. Crosses denote two-way ANOVA comparisons between the groups Ad-P1.7,16-prime, OMV-boost and OMV-prime, OMV-boost. ∗∗ or ++ = P ≤ 0.01, +++ = P ≤ 0.001 and ++++ = P ≤ 0.0001. Antibody responses to FetA F3–3 (B) Groups of 8 BALB/c mice were immunised with 10⁹ IU of adenoviral vectors Ad-F3–3, Ad-FliC-VR3–3 or 5 μg OMVs (44/76 FetA_on PorA_off), and then boosted with OMV-44/76 FetA_on PorA_off at week 8, vaccination time points are marked with arrows. ELISAs were performed against recombinant FetA rF3–3. Kruskal Wallis with Dunn’s multiple comparison tests were used to perform comparisons between week 6 vs. week 14 or week 20 for Ad-FliC-VR3–3 boosted with OMV (asterisks) or OMV boosted with OMV (crosses) + = P ≤ 0.05, ^∗∗ or ++ = P ≤ 0.01 and ^∗∗∗ = P ≤ 0.001