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. Author manuscript; available in PMC: 2024 Nov 11.
Published in final edited form as: Mol Cell. 2023 Oct 10;83(22):4017–4031.e9. doi: 10.1016/j.molcel.2023.09.029

Figure 2. Cryo-EM analysis of Cdc45 containing complexes leads to identification of a DONSON-bound double CMG assembly.

Figure 2

(A). The N-terminal end-on view, the side, and the rotated side view of single CMG.

(B). The side view of a double CMG in a configuration distinct from what was previously observed with the yeast CMG assembled at an origin of replication.

(C) A view of the MCM ATPase improved after focused classification and refinement.

(D) Unassigned density after building the double CMG atomic model.

(E) Cartoon representation of a DONSON homodimer built into the cryo-EM density represented with transparent surface rendering and a cut-through view.

(F) Mass photometry analysis demonstrates that purified recombinant DONSON homodimerizes.