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. Author manuscript; available in PMC: 2024 Nov 11.
Published in final edited form as: Mol Cell. 2023 Oct 10;83(22):4017–4031.e9. doi: 10.1016/j.molcel.2023.09.029

Figure 7. A model for the incorporation of two GINS factors into two CMG assemblies, mediated by dimeric DONSON.

Figure 7

Loaded MCM double hexamers are phosphorylated by DDK.

The phosphorylation is read by Treslin-MTBP, which in turn promote Cdc45 recruitment onto the MCM double hexamer. A DONSON dimer engages two copies of the GINS firing factors. It recruits them to the MCM double hexamer by engaging the Mcm3 ATPase via the D3 loop. The DONSON dimer then locks onto the double hexamer, delivering GINS to form the CMG. This leads to a reconfiguration in the double hexamer structure, with two MCM rings that rotate clockwise with respect to one another and become offset ready for replication fork establishment.