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. Author manuscript; available in PMC: 2024 Nov 19.
Published in final edited form as: Cell. 2024 Sep 13;187(21):5967–5980.e17. doi: 10.1016/j.cell.2024.08.024

Figure 4. Tau-RING prevents seeded aggregation in primary neurons.

Figure 4

(A) Schematic of the primary neuron seeding assay, using P301S tau transgenic mice at P2. Cultures were infected with AAV PHP.eB carrying venus-P2A-tau-RING (VPTR) or venus only at day in vitro 2 (DIV2), and P301S tau aggregates were added to the media at DIV7. Cultures were evaluated at DIV14 for the number of AT8-positive tau aggregates.

(B) Quantification of primary neurons treated ±100 nM tau aggregates, ±venus, or venus-P2A-tau-RING (VPTR) AAV at DIV14. N = 3.

(C) Representative immunofluorescence images of primary neuron cultures at DIV14, treated with venus or VPTR AAV, +100 nM P301S tau aggregates. Scale bar, 100 μm. Statistical significance for (B) determined by two-way ANOVA and Sidak’s multiple comparisons test. ****p < 0.0001. ns, non-significant.

See also Figure S3.