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. Author manuscript; available in PMC: 2024 Dec 5.
Published in final edited form as: Anal Chem. 2021 Feb 17;93(8):3686–3690. doi: 10.1021/acs.analchem.1c00257

Figure 3. Chromatographic stability of the same PepMap column over a 20 months interval.

Figure 3

(A) Boxplots showing the chromatographic peak width (full width at half-maximum; FWHM) distributions of Hela peptides separated on the same PepMap column with different gradients in 2018 (red) and 2020 (blue; Orbitrap HF-X). (B) Boxplots showing the chromatographic peak width (full width at half-maximum; FWHM) distributions of Hela peptides separated on the same column using 60 min gradient between 2018 to 2020. Boxes cover 50% and whiskers represent 1.5 times the interquartile range of the data. (C) Overlaid pressure curves of Hela peptides separated on the same column between 2018 to 2020 using a 60 min gradient.