Figure 3. CC_SHR self-assembly and folding.

(a) Cryo-TEM image of the nanofibers (scale bar 100 nm). (b) NH region ROESY spectrum showing sequential d_NN connectivity typical of a helical secondary structure (one letter notation of the amino acids: Phe, F; Aib, U; Leu, L; Glu, E. Residues are numbered in ascending order from N- to C-terminus). (c) Plot of chemical shift variations against temperature for α- and β-protons. (d) Temperature-dependent CD spectra. (e) Thermal unfolding profiles at different peptide concentrations. [(1) T_m = 40 °C (5 mg mL⁻¹); (2) T_m = 48 °C (7 mg mL⁻¹); (3) T_m = 47 °C (8.5 mg mL⁻¹)]. (f) Absorbance vs wavelength profile of nanofibers prepared at 7 mg mL⁻¹ concentration at different temperatures.