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. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Nat Metab. 2020 Jan 20;2(1):62–80. doi: 10.1038/s42255-019-0159-z

Extended Data Fig. 2. ECM stiffness directs creatine metabolism in PDAC cells.

Extended Data Fig. 2

(a) Schematic representation of the phosphocreatine circuit. Red indicates metabolite enrichment on soft (0.7kPa) ECM, while blue indicates enrichment on stiff (glass) ECM.

(b) Urea cycle and creatine biosynthesis metabolic intermediates of KPC cells cultured on fibronectin-coated 0.7-38 kPa hydrogels and glass coverslips as indicated. Values are mean ± SD from 3 biological replicates within the same day. Statistical significance assessed by one-way ANOVA.

(c) Urea cycle and creatine biosynthesis metabolic intermediates of PANC-1 cells cultured as indicated. Values are mean ±SD from 3 biological replicates within the same day. Statistical significance assessed by two-tailed unpaired t-test with Welch’s correction.

(d) Arginine-derived labelled carbon and nitrogen incorporation in urea cycle and creatine biosynthesis metabolites of KPC cells cultured as indicated and supplemented with L-arginine-13C615N4 for 1, 3 and 6 hours. Values are mean ±SD from 3 biological replicates within the same day.