Figure 2. Differentiation of neutrophil-like PLB-985 like is associated with enhanced LILRA6/B3 surface expression.

(A) Undiffereniated PLB-985 cells express low levels of LILRA6/B3. PLB-985 cells were cultured in RPMI 1640 at 37°C with 5% CO₂, and stained with primary antibodies. Anti-IgG-PE was used as a secondary mAb for anti-LILRB3 and IgG2a isotype control. A representative experiment from 3 sperate experiments is shown. (B, C and D) DMSO-differentiated PLB-985 cells expressed enhanced surface levels of LILRA6/B3. PLB-985 cells were cultured in the presence or absence of 1.25% DMSO at 37°C with 5% CO₂ for up to 5 days. Cells were stained with mAb and fluouresence was measured by flow cytometry. Integrated results from three separate experiments (B) were compared by Student t-test, where * = p < 0.05, ** = p < 0.01. Data from a representative experiment are shown (C). Relative receptor expression on DMSO-differentiated PLB-985 populations to undifferentiated PLB-985 populations (D) was plotted, where Student t test was used to compare the relative receptor expression between day 0 and day 5.