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. Author manuscript; available in PMC: 2024 Dec 9.
Published in final edited form as: Immunity. 2021 Oct 12;54(11):2531–2546.e5. doi: 10.1016/j.immuni.2021.09.010

Figure 2. IL-33 triggers the IL-4-independent differentiation of AAMs.

Figure 2

(A–D) Bulk mRNA sequencing data comparing BMDMs stimulated with vehicle (Ctrl), IL-33 (10 ng/mL for 36 h), and IL-4 (20 ng/mL for 36 h). Data are presented as a heatmap illustrating differential gene expression (A), PCA plot (B), volcano plot (C), and heatmap of genes for alternative and classic macrophage activation derived from bulk sequencing data in the different conditions (D).

(E–I) Quantitative real-time PCR (E and G), immunoblot (F and H), and ELISA (I) showing mRNA and protein expression of indicated markers of alternative activation (Arg1, Retnla, Chil3, ARG1) and classic activation (TNF) in BMDMs of Il4raWT and Il4raΔmac (E and F) or Myd88+/− and Myd88−/− (G–I) upon stimulation with vehicle (Ctrl) or IL-33 (10 ng/mL for 5 days) or IL-4 (20 ng/mL for 24 h). Data are representative of two individual experiments. Not detected (n.d.).

Data are presented as mean + SEM. *p < 0.05, **p < 0.01, and ***p < 0.001.