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(A) Immunostaining of phosphorylated HSL (red) in paraffin sections of epididymal fat of C57Bl6/J mice that were peripherally administrated with 500 ng/h recombinant leptin for 2 days. (B) Phosphorylated HSL and phosphorylated PKA substrates in total protein extracts of epididymal fats were examined by immunoblot analysis.
