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. Author manuscript; available in PMC: 2024 Dec 10.
Published in final edited form as: Cell. 2015 Sep 24;163(1):84–94. doi: 10.1016/j.cell.2015.08.055

Figure 2. Neural Projections in Fat Detected with Optical Projection Tomography.

Figure 2

(A) Schematic representation of the OPT method applied to the subcutaneous inguinal fat. (i) Tissue dissection. (ii) Sample clearing. (iii) Image acquisition. (iv) Sinogram transformation. (v) 3-D reconstruction and segmentation. (B) OPT series of coronal sections of inguinal fat organ after 3-D reconstruction. (C) Orthogonal 400 μm OPT-slabs of inguinal fat in coronal, axial and sagittal view. Axon budles were identified based on the grey threshold level (arrows). (D) 3-D reconstruction in maximal intensity projection of the OPT coronal sections. (E) Surface view of segmented structures within inguinal fat.