Skip to main content
. Author manuscript; available in PMC: 2024 Dec 10.
Published in final edited form as: Cell. 2015 Sep 24;163(1):84–94. doi: 10.1016/j.cell.2015.08.055

Figure 5. Sympathetic Neurons are Locally Required for Leptin-induced Lipolysis.

Figure 5

C57BL6/J mice were peripherally administrated with 500 ng/h recombinant leptin or saline for 2 days. (A) NE content in subcutaneous fat pads (*p<0.05, n=5) and (B) NE serum levels were measured by NE ELISA (n=4). Results are shown as mean ± SEM. C57BL6/J mice were peripherally administrated with 500 ng/h recombinant leptin at 3 days after local crush injury of nerves in fat pads. (C) Phosphorylated HSL in total protein extracts of epididymal fats were examined by immunoblot analysis. (D) Fat pads in TH-Cre; LSL-DTR mice were locally treated with DT. Tissue specific ablation of SNS axons confirmed by immunostaining for Tub-3 and TH (**p< 0.001, n=6). Results are shown as mean ± SEM. (E) Immunoblot analysis of phosphorylated HSL in total protein extracts of subcutaneous fats of TH-Cre; LSL-DTR and control mice injected with DT, following leptin treatment (500 ng/h). See also Figure S1.