Figure 13.
Doxorubicin drug cytotoxicity screening assay performed in 3D spheroids at 7 days of culture. (A) A549 monoculture spheroids. (B) Dual coculture spheroids A549:HF. (C) tri-coculture spheroids of A549:HF:hBM-MSCs. (D) Cell viability data of all tested conditions at the highest administered dose of Doxorubicin (17 μM). Different colors represent: 3D-MCTS (control blue bars), 3D-MCTS with non-treated PCL microparticles (NT-MPs) (grey bars) and 3D-MCTS with LbL-MPs (green bars). Data is presented as mean ± s.d. (n= 6). * p<0.05, **p<0.01 and ***p<0.001. ns – represents non-significant differences.