Figure 7.
Histological analysis of A549:HF:hBM-MSCs tri-coculture spheroids. (A, B) Spheroids with and without LbL-MPs. (C, D) Internal organization of cells around MPs. Image A, with cells clearly adhering to the surface of LbL-MPs and acquiring an elongated shape (A – red arrows), furthermore a possible necrotic region is observed (red circle). (C and D) At day 7 ECM deposition is already visible in both spheroids occurring however more markedly in tri-coculture spheroids with LbL-MPs (blue arrows and circles). Scale bar = 50 μm.