Kinetics of IκBα and β expression in TsAg-stimulated human monocytes. Human monocytes were stimulated with either media (control) or TsAg (100 μg/ml) for 0, 5, 10, 15, 30, 60, 120 and 240 min. Cytoplasmic lysates were extracted and equal amounts of protein separated by SDS-PAGE before being analysed by Western blotting using antibodies specific to (A) IκBα and (B) IκBβ. Blots shown are representative of experiments performed on three separate occasions.